GAPDH is Determined to be a Useful Control Antibody for Studying Sickle Cell Disease in Livers

Abstract

Using the Western blot procedure, a reliable control antibody was determined to be GAPDH when studying Sickle Cell Disease (SCD). Three primary antibodies were tested for their consistency in determining protein concentration in sickle cell mouse livers. As hypothesized, GAPDH accurately expressed protein concentration within the samples. Beta Actin was a less fit antibody because of its high presence within cytoskeletal filaments, which are lost during hemolysis of cells. KCTD12 was partially successful, likely due to its high presence in liver Kupffer cells. Determining GAPDH as a reliable primary antibody, the presence of desired proteins can be studied to monitor liver health in SCD patients.