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    When is a Bug a Feature? : Enzymatic Activity in the Beer Brewing Process

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    BirchSpr22-2.pdf (599.2Kb)
    Date
    2022-04
    Author
    Birch, Cole M.
    Leavens, Caterra M.
    Sillman, Hunter W.
    Voon, Aidan
    Bailey-Hartsel, Scott
    Metadata
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    Abstract
    The beer brewing industry has grown rapidly over the past several years. Because of this, the number of hop varieties has greatly expanded, making them a very valuable resource. To alleviate the financial strain, a beta-glucosidase enzyme product (X-zyme) has been created with the intention to reduce the amount of hops used in the brewing process while maintaining the levels of aroma and flavor. However, in previous studies, Xzyme has displayed potential esterase activity, producing undesirable phenolic off-flavors (POFs). To study this esterase activity of X-zyme, a spectrophotometric assay was designed using para-nitrophenyl ferulate. Detected esterase activity could indicate that X-zyme has potential off-target effects that could lead to the dumping of an entire load of beer, a significant financial loss. The assay showed significant feruloyl esterase activity that may have an impact on the conditions X-zyme should and should not be used.
    Subject
    Breweries
    Enzymes
    Esterases
    Posters
    Department of Chemistry and Biochemistry
    Permanent Link
    http://digital.library.wisc.edu/1793/83733
    Description
    Color poster with text, images, charts, and graphs.
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    • Student Research Day

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