In the present study, we have employed site-directed mutagenesis to probe the role of four residues in E. coli ProRS (Ec ProRS) on the overall catalytic function. Specifically, we examined the impact of changes in amino ...

Aminoacyl-tRNA synthetases (AARSs) are enzymes that catalyze the covalent attachment of amino acids to their cognate tRNA. This reaction is known as aminoacylation of tRNA and is crucial for protein synthesis in all living ...

Around the world, cancer is one of the leading causes of death. DNA is the primary target molecule for most anticancer therapy. Transition metal complexes are known to have DNA binding and cleavage properties under ...

Cancer now tops heart disease as the number one cause of death in the United States and is the second leading cause of death globally, accounting for an estimated 9.6 million deaths in 2018. There is an urgent need for ...

Poly(ethylene glycol) (PEG) is a biocompatible, hydrophilic, flexible, non-toxic polyether commonly used in proteomics because it is widely regarded to be a biologically inert molecule. This includes PEG2000 (molecular ...

To explore the structure-dynamics-function relationship in proteins, most studies are usually conducted in simple salt-buffer solutions, however, the interior of a cell is densely crowded. The concentration of macromolecular ...

Amino-acyl tRNA synthetases (AARSs) belong to a class of an important family of enzymes that are critical for proteins biosynthesis in all living organisms. They catalyze aminoacylation of tRNAs, a key step in protein ...

Fluorescence spectroscopy has become a pivotal tool in biochemical research by virtue of its robustness and high sensitivity. Intrinsic protein fluorescence, that originates mainly from the aromatic amino acid tryptophan, ...

This research project is a continuing effort focused on the molecular-level understanding of the relationship between structure, dynamics, and function in aminoacyl-tRNA synthetases (AARSs). Recent computational studies ...