Now showing items 1-6 of 6
Investigating Gene-Environment Interactions of Methylmercury Using Zebrafish
Methylmercury (MeHg) is an environmental pollutant found in the Great Lakes and a known danger to prenatal health. The upper limit considered to be safe is 5.8 ppb for newborns in a blood spot taken at birth. In the Lake ...
Investigating Autism Spectrum Disorder through Environmental Contaminants : Effects of Lead Exposure on Zebrafish Development
Lead is an extremely toxic heavy metal that is known to causes curvature in zebrafish larvae neurodevelopmental damage. Lead exposure can impair neurodevelopment and is correlated to an increased prevalence of Autism ...
Assessing Efficiency of qPCR primers for Neuronal Marker Genes in Zebrafish
Quantitative PCR (qPCR) is used to measure mRNA levels and has been broadly used to measure gene expression in zebrafish. Previous studies have shown the value of qPCR in time-course experiments for tracking the expression ...
High-Resolution Melt Analysis Can Distinguish Variable PCR Products for Genotyping CRISPR Mutagenesis in Zebrafish
Seventy percent of human genes have at least one ortholog found in the zebrafish genome, providing a useful vertebrate model to study gene function. The CRISPR/Cas9 system combined with microinjection is a well-known method ...
Ethanol Exposure Alters Motor Abilities in Zebrafish Embryos
Fetal Alcohol Syndrome (FAS) is a condition that can affect the physical health and behavior of developing fetuses as a result of the pregnant mother’s consumption of alcohol. FAS is found in 0.2-1.5 infants out of 1,000. ...
Methylene Chloride Exposure Alters Morphological Development in Zebrafish Larvae
The prevalence of individuals diagnosed with Autism Spectrum Disorder (ASD) has strikingly increased in recent decades. Both genetic and environmental factors have been associated with neurodevelopmental changes causing ...