Search

Now showing items 1-4 of 4

Studying the Interactions between Monosaccharide / Polysaccharide with Tryptophan Using Fluorescence Spectroscopy

Johnson, Benjamin; Bhattacharyya, Sudeep; Hati, Sanchita (2019)

Fluorescence spectroscopy has become a pivotal tool in biochemical research by virtue of its robustness and high sensitivity. Intrinsic protein fluorescence, that originates mainly from the aromatic amino acid tryptophan, ...

Site-Directed Mutagenesis and Intrinsic Tryptophan Fluorescence Study to Probe the Conformational Change in Escherichia coli Prolyl-tRNA Synthetase (Ec ProRS)

Weeks, Katelyn; Adams, Lauren; Weinzetl, Murphi; Liebau, Jessica; Hati, Sanchita; Bhattacharyya, Sudeep (2019-05)

Amino-acyl tRNA synthetases (AARSs) belong to a class of an important family of enzymes that are critical for proteins biosynthesis in all living organisms. They catalyze aminoacylation of tRNAs, a key step in protein ...

Exploring the Effects of Monomer and Polymer Cosolutes on the Conformation of Escherichia coli Prolyl-tRNA Synthetase Using Intrinsic Tryptophan Fluorescence Spectroscopy

Johnson, Benjamin; Fossum, Carl; Liebau, Jessica; Liew, Aubrey; Laatsch, Bethany; Hati, Sanchita (2021-04)

How Does Varying Sizes and Concentrations of Poly(Ethylene Glycol) Affect the Conformation and Function of the Enzyme Escherichia Coli Prolyl-Trna Synthetase?

Liebau, Jessica; Laatsch, Bethany; Lowater, Harrison; Hati, Sanchita (2021-04)

Poly(ethylene glycol) (PEG) is a biocompatible, hydrophilic, flexible, non-toxic polyether commonly used in proteomics because it is widely regarded to be a biologically inert molecule. This includes PEG2000 (molecular ...