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Stable expression and purification of Juvenile Hormone Binding Protein from Drosophila melanogaster Schneider line-2 cells
Studies on the hemolymph Juvenile Hormone Binding Protein (hJHBP) and Juvenile Hormone (JH) interaction have been considerably limited by the availability of pure hJHBP. An expression vector was created by cloning the ...
Analyzing interactions between the GV1 Cell Line, Juvenile Hormone, and Juvenile Hormone Binding Protein
Insect juvenile hormone (JH) and its hemolymph transport protein, juvenile hormone binding protein (JHBP) play key roles in the development of many insects. However, the exact mechanism via which JH and JHBP regulates ...
Herbivory defense in Mimulus quttatus (yellow monkeyflower): consequences for generalist and specialist herbivores
Plants have undergone strong selection from herbivory, resulting in diverse physical and chemical defenses. Chemical defenses deter generalist herbivores, but are often tolerated by specialists. Mimulus guttatus (yellow ...
Large scale production of stable hemolymph juvenile hormone binding protein
To better understand the pathways of the insect juvenile hormone (JH) action, milligram amounts of pure hemolymph juvenile hormone binding protein (hJHBP) are required. Hence the goal of this study is to scale up the system ...
The effects of the Anopheles gambiae CLIPC3 and CLIPC1 genes on melanization of an abiotic target
Melanization is a mechanism of innate immunity in arthropods wherein a thick capsule of melanin is formed around foreign objects. Little is known about this pathway biochemically though serine proteases are known to ...